Journal: Biomaterials Research

Article Title: Oxidative Stress and Hormone-Regulated Dermal Papilla Cell-Targeted Nanomodulators: Reverse Cellular Senescence for Androgenetic Alopecia Therapy

doi: 10.34133/bmr.0333

Figure Lengend Snippet: LEPR expression in DPCs and validation of binding to leptin. (A) LEPR expression in HDPCs, HaCaT cells, MDPCs, and mEK cells. Scale bar = 50 μm. (B) Quantitative analysis of LEPR expression in cells ( n = 3). (C) Time scale analysis of the HF cycle in C57BL/6 mice at 11 weeks after birth. (D) LEPR expression in different HF phases of mice. Scale bar = 25 μm. (E and F) Western blotting and grayscale analysis of LEPR expression (HDPC and HaCaT) ( n = 3). (G) The mechanism by which leptin targets LEPR-expressing DPCs. (H) CLSM imaging following co-incubation with FITC-labeled leptin (green) and Alexa Fluor 647-labeled LEPR (red). Scale bar = 20 μm. (I) Grayscale colocalization analysis of the images shown in (H), (a) PCC (HDPC) = 0.5298 and (b) PCC (MDPC) = 0.5263. All results are presented as means ± SDs (* P < 0.05 and *** P < 0.001). DAPI, 4′,6-diamidino-2-phenylindole; HF, hair follicle; HDPC, human dermal papilla cell; MDPC, mouse dermal papilla cell; mEK, mouse epidermal keratinocyte; FITC, fluorescein isothiocyanate; PCC, Pearson correlation coefficient.

Article Snippet: Following a 24-h incubation, the mouse skin was subjected to cryosectioning and subsequently labeled with a rabbit monoclonal antibody specific to LEPR proteins (dilution 1:100; HUABIO), followed by incubation with an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody.

Techniques: Expressing, Biomarker Discovery, Binding Assay, Western Blot, Imaging, Incubation, Labeling